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Issue 2, 2011
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DNAdetection of chronic myelogenous leukemia by magnetic nanoparticles

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Abstract

A novel tool for the detection of BCR/ABL fusion gene in chronic myelogenous leukemia (CML) was developed by a magneto-polymerase chain reaction (PCR)-enzyme linked gene technique. The forward primers covalently bound to the surface of magnetic nanoparticles allowed a convenient separation of PCR products with high sensitivity (0.5 pg ml−1) and high specificity using K562 cell line and CML patients. The results were obtained when the biotinylated-reverse primer bound to streptavidin-horseradish peroxidase (HRP) and hydrolysed the substrate. This novel readout system was approximately 1000-fold more sensitive than the conventional agarose gel electrophoresis. The present technique is practical and useful for following up CML patients and for providing appropriate treatment, particularly to patients in remote areas.

Graphical abstract: DNA detection of chronic myelogenous leukemia by magnetic nanoparticles

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Publication details

The article was received on 04 Jun 2010, accepted on 19 Sep 2010 and first published on 21 Oct 2010


Article type: Paper
DOI: 10.1039/C0AN00374C
Analyst, 2011,136, 354-358

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    DNA detection of chronic myelogenous leukemia by magnetic nanoparticles

    K. Jangpatarapongsa, D. Polpanich, V. Yamkamon, Y. Dittharot, J. Peng-On, R. Thiramanas, S. Hongeng, S. Jootar, L. Charoenmak and P. Tangboriboonrat, Analyst, 2011, 136, 354
    DOI: 10.1039/C0AN00374C

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