Issue 1, 2009
From the journal:

Analyst

Rapid cell mapping using nanoparticles and SERRS

Robert J. Stokes,a   Fiona McKenzie,a   Emma McFarlane,b   Alastair Ricketts,a   Laurence Tetley,c   Karen Faulds,a   James Alexanderb  and  Duncan Graham*a  

* Corresponding authors

a Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UK
E-mail: duncan.graham@strath.ac.uk
Tel: +44(0)141 548 4701

b Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 27 Taylor Street, Glasgow, UK

c Division of Infection & Immunity, IBLS, Joseph Black Building, University of Glasgow, Glasgow, UK

Abstract

Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.

Graphical abstract: Rapid cell mapping using nanoparticles and SERRS

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Article information

DOI
https://doi.org/10.1039/B815117B
Article type
Paper
Submitted
01 Sep 2008
Accepted
29 Oct 2008
First published
19 Nov 2008

Analyst, 2009,134, 170-175

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Rapid cell mapping using nanoparticles and SERRS

R. J. Stokes, F. McKenzie, E. McFarlane, A. Ricketts, L. Tetley, K. Faulds, J. Alexander and D. Graham, Analyst, 2009, 134, 170 DOI: 10.1039/B815117B

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