Issue 1, 2009
From the journal:

Analyst

Using acridinium ester as the sonochemiluminescent probe for labeling of protein

Yongquan Lai,a   Yuanyuan Qi,a   Jian Wang*a  and  Guonan Chen*a  

* Corresponding authors

a Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), and Department of Chemistry, Fuzhou University, Fuzhou, Fujian, China
E-mail: gnchen@fzu.edu.cn, jwang@fzu.edu.cn
Fax: +86 591-83713866
Tel: +86 591-87893315

Abstract

A new sonogenerated chemiluminescence (SCL) system has been developed to investigate the SCL behavior of acidinium ester (AE). The optimum parameters for this SCL system have also been investigated in detail. Under the optimum conditions, a typical SCL signal could be obtained in a mixture of DMF and alkaline water solutions with volume ratio of 3 : 2. According to electrospray ionization mass spectrometry and sonochemiluminescence emission spectra, SCL emission could be ascribed to the formation of N-methylacridone with a different mechanism from that in both the chemiluminescence and electrochemiluminescence reaction of acridinium ester. The acridinium ester has been used as a SCL probe for labeling of anti-human insulin guinea pig polyclonal antibody, and it was found that the concentration of AE-conjugated antibody was linear with the SCL intensity in the range of 0.15–0.75 ppb with a linear correlation coefficient of 0.9980.

Graphical abstract: Using acridinium ester as the sonochemiluminescent probe for labeling of protein

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Article information

DOI
https://doi.org/10.1039/B813330A
Article type
Paper
Submitted
01 Aug 2008
Accepted
03 Sep 2008
First published
20 Oct 2008

Analyst, 2009,134, 131-137

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Using acridinium ester as the sonochemiluminescent probe for labeling of protein

Y. Lai, Y. Qi, J. Wang and G. Chen, Analyst, 2009, 134, 131 DOI: 10.1039/B813330A

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