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Issue 9, 2008
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Self-assembled multilayer of gold nanoparticles for amplified electrochemical detection of cytochromec

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Abstract

Although different kinds of film materials and some modification techniques are applied for the development of protein-film electrochemistry, the design of a more ordered adsorption platform with improved sensitivity is still required. Here we employ single-strand DNA (ssDNA)-functionalized gold nanoparticles as scaffolds for the construction of a multilayered uniform self-assembled structure via the hybridization of complementary ssDNA. After adsorbing with native conformation onto the uniformly built electrode, cytochrome c responded very well in voltammetry experiments. The peak currents increase with the addition of the number of gold nanoparticle layers, which indicates that the multilayer gold nanoparticles not only provide a compatible microenvironment for the protein to undergo direct electron transfer reactions but also amplify the electrochemical signals by increasing the binding sites for the protein immobilization. Furthermore, ultra-sensitive detection of cytochrome c by using this multilayer gold nanoparticle-modified electrode is carried out. The linear range is from 2 × 10−9 to 1 × 10−7 M with a detection limit of 6.7 × 10−10 M.

Graphical abstract: Self-assembled multilayer of gold nanoparticles for amplified electrochemical detection of cytochrome c

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Article information


Submitted
18 Apr 2008
Accepted
03 Jul 2008
First published
25 Jul 2008

Analyst, 2008,133, 1242-1245
Article type
Paper

Self-assembled multilayer of gold nanoparticles for amplified electrochemical detection of cytochrome c

J. Zhao, X. Zhu, T. Li and G. Li, Analyst, 2008, 133, 1242
DOI: 10.1039/B806588H

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