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Issue 10, 2008
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Isolation and pre-concentration of basic proteins in aqueous mixture viasolid-phase extraction with multi-walled carbon nanotubes assembled on a silica surface

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Abstract

Multi-walled carbon nanotubes (MWNTs) were assembled on a silica surface using a polyelectrolyte-assisted layer-by-layer (LBL) assembly technique. The surface-assembled silica spheres with MWNTs (MWNTs/SiO2), which serve as a novel solid-phase extraction sorbent for separation/pre-concentration of basic proteins, was investigated. The adsorption behavior of cytochrome c (cyto-c) by MWNTs/SiO2 spheres agrees well with the Langmuir adsorption model. A thorough scrutiny of the experimental parameters affecting the adsorption of cyto-c from aqueous solution onto the MWNTs/SiO2 spheres and its subsequent desorption was carried out. A maximum adsorption capacity of cyto-c was derived as 112 mg (cyto-c) g−1 (MWNTs). A distinct feature of the MWNTs/SiO2-packed micro-column provides clear advantages of minimized flow impedance when operated in a flow system, in addition to better separation efficiency as well as the favorable enrichment capability of proteins, characterized by an enrichment factor of 30 by using 2.0 mL of aqueous solution. The practical applicability of the MWNTs/SiO2 spheres as a sorbent for the isolation of basic proteins from acidic protein species was demonstrated by effective separation of cyto-c from bovine serum albumin (BSA).

Graphical abstract: Isolation and pre-concentration of basic proteins in aqueous mixture viasolid-phase extraction with multi-walled carbon nanotubes assembled on a silica surface

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Publication details

The article was received on 13 Mar 2008, accepted on 03 Jun 2008 and first published on 11 Jul 2008


Article type: Paper
DOI: 10.1039/B804347G
Analyst, 2008,133, 1373-1379

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    Isolation and pre-concentration of basic proteins in aqueous mixture viasolid-phase extraction with multi-walled carbon nanotubes assembled on a silica surface

    Z. Du, Y. Yu, X. Yan and J. Wang, Analyst, 2008, 133, 1373
    DOI: 10.1039/B804347G

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