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Issue 7, 2007
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Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies

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Abstract

Surface enhanced Raman scattering (SERS) has been used to detect bacteria captured by polyclonal antibodies sorbed onto protein-A-modified silver nanoparticles. The selectivity and discrimination of the technique were assured by using a specific antibody to the model bacterium, Escherichia coli. As the SERS enhancement mechanism depends upon the metal surface proximity, 8 nm was considered as the optimum distance between the bacterium and the nanoparticle surface. Spectral reproducibility was verified using Principal Components Analysis to differentiate the clusters corresponding to the biomolecules and/or bacteria sorbed onto nanoparticles. Compared to the normal Raman spectrum, the SERS technique resulted in an intensity enhancement of over 20-fold.

Graphical abstract: Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies

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Publication details

The article was received on 25 Jan 2007, accepted on 17 May 2007 and first published on 05 Jun 2007


Article type: Paper
DOI: 10.1039/B701160A
Analyst, 2007,132, 679-686

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    Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies

    G. Naja, P. Bouvrette, S. Hrapovic and J. H. T. Luong, Analyst, 2007, 132, 679
    DOI: 10.1039/B701160A

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