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Issue 9, 2005
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Development of a micro dual beam fluorometric detector specific for microchip analysis of benzo[a]pyrene and benzo[k]fluoranthene in diesel exhaust particulate samples

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Abstract

We developed a device and some systems for detecting benzo[a]pyrene (B[a]P) and benzo[k]fluoranthene (B[k]F). The device uses a UV light-emitting diode that emits light with a wavelength of 370 nm and a violet laser diode that emits light with a wavelength of 395 nm as excitation light sources. The detection method is based on the following observation: characteristic fluorescence is emitted from both B[a]P and B[k]F, with intensities up to 10 times greater than those from 22 other polycyclic aromatic hydrocarbons (PAHs). The excitation and emission wavelengths for fluorescence from B[a]P and B[k]F are 370 and 429 nm, and 395 and 429 nm, respectively. Further, we calculated their concentrations in diesel exhaust particles by means of two calibration curves determined using a mixture of 24 PAHs. The detection limits of the proposed device with a microchip are 5.58 µg L−1 for B[a]P and 6.03 µg L−1 for B[k]F. We applied this method to analyze B[a]P or B[k]F contained in three types of diesel exhaust particles, and the results agreed well with those obtained by liquid chromatography.

Graphical abstract: Development of a micro dual beam fluorometric detector specific for microchip analysis of benzo[a]pyrene and benzo[k]fluoranthene in diesel exhaust particulate samples

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Article information


Submitted
30 Mar 2005
Accepted
22 Jun 2005
First published
07 Jul 2005

Analyst, 2005,130, 1253-1257
Article type
Paper

Development of a micro dual beam fluorometric detector specific for microchip analysis of benzo[a]pyrene and benzo[k]fluoranthene in diesel exhaust particulate samples

H. Masaki, H. Susaki and T. Korenaga, Analyst, 2005, 130, 1253
DOI: 10.1039/B504440E

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