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Issue 5, 2005
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Evaluation of the antioxidant activity by flow injection analysis method with electrochemically generated ABTS radical cation

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Abstract

A Trolox equivalent antioxidant capacity (TEAC) decolourisation assay was adapted to a flow injection analysis (FIA) system and a simple and rapid method for antioxidant activity evaluation was developed. To avoid the time consuming step of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation preparation by chemical oxidation of ABTS, as in the original TAEC assay, and hence, to shorten the analysis time, the ABTS radical cation was generated on-line by electrochemical oxidation of ABTS in the flow-through electrolysis cell forming a part of the FIA system. The proposed method was optimised with respect to a flow rate, injection volume and ABTS radical cation/carrier ratio. Under the optimised conditions linear calibration graphs for Trolox were obtained over the range 10–100 µM, with a limit of detection 1.6 µM. Good reproducibility (relative standard deviation 1.95%) and sample throughput (32 samples per hour) were achieved. The developed method was applied to the evaluation of the antioxidant activity of pure compounds and samples of some common beverages. In both cases a good correlation between the results obtained by the proposed method and TEAC values evaluated by the classic TAEC decolourisation assay was obtained (r2 = 0.996 for pure compounds and r2 = 0.957 for beverage samples).

Graphical abstract: Evaluation of the antioxidant activity by flow injection analysis method with electrochemically generated ABTS radical cation

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Publication details

The article was received on 14 Oct 2004, accepted on 25 Feb 2005 and first published on 15 Mar 2005


Article type: Paper
DOI: 10.1039/B415939J
Citation: Analyst, 2005,130, 708-714

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    Evaluation of the antioxidant activity by flow injection analysis method with electrochemically generated ABTS radical cation

    D. Iveković, S. Milardović, M. Roboz and B. S. Grabarić, Analyst, 2005, 130, 708
    DOI: 10.1039/B415939J

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