Issue 10, 2004

Sub-picomole colorimetric single nucleotide polymorphism discrimination using oligonucleotide–nanoparticle conjugates

Abstract

Oligonucleotidegold nanoparticle conjugates are employed to demonstrate selective colorimetric discrimination of a cystic fibrosis-related genetic mutation in a model oligonucleotide system. Specifically, three strand oligonucleotide complexes are employed, wherein two probe oligonucleotidegold nanoparticle conjugates are linked together by a third target oligonucleotide strand bearing the chosen CF-related mutation. By monitoring the solution optical absorption behaviour of the complexes as a function of temperature, melting profiles may be accurately acquired and reproducibly compared. Following this method, fully complementary sequences are successfully distinguished from mismatched sequences, with single base mismatch resolution, for the V232D mutation. To extend the detection sensitivity of this oligonucleotidenanoparticle conjugate assay, a novel and compact LED-based optical monitoring system capable of sub-picomole level colorimetric SNP discrimination is also demonstrated.

Article information

Article type
Paper
Submitted
03 Jun 2004
Accepted
27 Jul 2004
First published
13 Aug 2004

Analyst, 2004,129, 970-974

Sub-picomole colorimetric single nucleotide polymorphism discrimination using oligonucleotidenanoparticle conjugates

D. Murphy, P. O'Brien and G. Redmond, Analyst, 2004, 129, 970 DOI: 10.1039/B408394F

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