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Issue 9, 2003

Rapid method for the preparation of a robust optical pH sensor

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Abstract

A simple and rapid method for the preparation of a fluorescence-based optical pH sensor is described. The sensor is based on excitation ratiometric detection of a methacryloyl-modified analog of the well-known fluorescent pH indicator dye, 8-hydroxy-1,3,6-pyrene trisulfonic acid (HPTS). The modified dye, 6-methacryloyl-8-hydroxy-1,3-pyrene disulfonic acid (MA-HPDS), is similar in structure and function to HPTS. However, unlike HPTS, the presence of the methacrylate moiety allows MA-HPDS to participate as a comonomer in a free radical polymerization reaction. Covalent immobilization by this method is simple, as the preparation of the modified dye is accomplished in a single reaction step and its subsequent reaction with a comonomer results in simultaneous immobilization and purification. Sensors were prepared by copolymerization of the MA-HPDS with poly(ethylene glycol) diacrylate. Minimal leaching of the immobilized dye was observed from the HPDS–PEG matrix. The copolymer of HPDS and PEG-DA is fully autoclavable. The sensor is useful over the pH range of 6–9, with excellent reproducibility. Ionic strength effects on the apparent pKa of the immobilized dye are small and predictable.

Article information


Submitted
01 May 2003
Accepted
04 Aug 2003
First published
19 Aug 2003

Analyst, 2003,128, 1181-1186
Article type
Paper

Rapid method for the preparation of a robust optical pH sensor

H. R. Kermis, Y. Kostov and G. Rao, Analyst, 2003, 128, 1181 DOI: 10.1039/B304902G

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