Issue 6, 2003

Comprehensive coupled reversed-phase reversed-phase separations of a complex isomeric mixture

Abstract

Using predictions based on results obtained from Information Theory and Factor Analysis for the two-dimensional separation of a complex isomeric mixture, a practical experimental comprehensive coupled reversed phase-reversed phase chromatographic system was developed. In total four reversed phase-reversed phase systems were studied, each of which theory predicted would be able to resolve essentially equal numbers of components. However, in practice only one of these coupled systems realised the theoretical potential. This system employed as the first dimension, a C18 stationary phase with methanol as the mobile phase and as the second dimension, carbon clad zirconia as the stationary phase and acetonitrile as the mobile phase. In this system, 27 of the 32 isomers of a mixture of oligostyrenes were resolved. Failure of the remaining coupled systems to achieve the theoretical potential was attributed to high solute crowding, low efficiency of separation space utilisation and long analysis times in the second dimension.

Article information

Article type
Paper
Submitted
03 Jan 2003
Accepted
18 Feb 2003
First published
10 Mar 2003

Analyst, 2003,128, 598-604

Comprehensive coupled reversed-phase reversed-phase separations of a complex isomeric mixture

M. J. Gray, A. P. Sweeney, G. R. Dennis, P. J. Slonecker and R. A. Shalliker, Analyst, 2003, 128, 598 DOI: 10.1039/B212742C

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