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Issue 12, 2002
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Competitive immunoassay for vancomycin using capillary electrophoresis with laser-induced fluorescence detection

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Abstract

A competitive immunoassay using capillary electrophoresis with laser-induced fluorescence was developed for vancomycin. Capillary electrophoresis using a Tris–glycine running buffer provided adequate separation of the antibody-bound from the unbound fluorescent probe (tracer) in less than 4 min. Laser-induced fluorescence polarization (LIFP) provided high sensitivity detection and simultaneous monitoring of fluorescence intensity and polarization. A fluorescence polarization value of 0.30 confirmed the formation of the antibody–tracer complex. Calibration curves showed a working linear range of 2–3 orders of magnitude with a minimum detectable concentration of 0.98 ng mL−1 (or 1.1 fg vancomycin). Clinical samples obtained from patients undergoing vancomycin treatment were analyzed for vancomycin and the results correlated well with a standard immunoassay based on latex particle detection that was routinely used by a hospital laboratory. Only 1/10 of the reagents were needed as compared with the standard immunoassay.

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Publication details

The article was received on 05 May 2002, accepted on 20 Oct 2002 and first published on 11 Nov 2002


Article type: Paper
DOI: 10.1039/B206531B
Citation: Analyst, 2002,127, 1633-1637

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    Competitive immunoassay for vancomycin using capillary electrophoresis with laser-induced fluorescence detection

    M. T. Lam and X. C. Le, Analyst, 2002, 127, 1633
    DOI: 10.1039/B206531B

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