Issue 11, 2001

Development and validation of a method for the confirmation of nicarbazin in chicken liver and eggs using LC-electrospray MS-MS according to the revised EU criteria for veterinary drug residue analysis

Abstract

A method is described for the quantitative confirmation of 4,4′-dinitrocarbanilide (DNC), the marker residue for nicarbazin in chicken liver and eggs. The method is based on LC coupled to negative ion electrospray MS-MS of tissue extracts prepared by liquid–liquid extraction. The [M–H] ion at m/z 301 is monitored along with two transition ions at m/z 137 and 107 for DNC and the [M–H] ion at m/z 309 for the internal standard, d8-DNC. The method has been validated according to the new EU criteria for the analysis of veterinary drug residues at 100, 200 and 300 μg kg−1 in liver and at 10, 30 and 100 μg kg−1 in eggs. Difficulties concerning the application of the new analytical limits, namely the decision limit (CCα) and the detection capability (CCβ) to the determination of DNC in both liver and eggs are discussed.

Article information

Article type
Paper
Submitted
13 Nov 2000
Accepted
24 May 2001
First published
03 Oct 2001

Analyst, 2001,126, 1985-1989

Development and validation of a method for the confirmation of nicarbazin in chicken liver and eggs using LC-electrospray MS-MS according to the revised EU criteria for veterinary drug residue analysis

S. Yakkundi, A. Cannavan, C. T. Elliott, T. Lovgren and D. G. Kennedy, Analyst, 2001, 126, 1985 DOI: 10.1039/B009084K

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