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Issue 2, 2000
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Determination of thiamine and riboflavin in pet foodsand animal feedingstuffs

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A method is given for the determination of thiamine (vitamin B1) and riboflavin (vitamin B2) in pet foods and animal feedingstuffs. The vitamins are extracted from the food matrix by digestion with 0.1 M hydrochloric acid followed by enzyme digestion with clara-diastase. The filtered solution is analysed by reversed-phase HPLC for riboflavin (directly) and thiamine (after thiochrome formation) using fluorescence detection.

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Publication details

The article was received on 30 Nov 1999 and first published on 03 Feb 2000

Article type: Paper
DOI: 10.1039/A909461J
Citation: Analyst, 2000,125, 353-360

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    Determination of thiamine and riboflavin in pet foods and animal feedingstuffs

    Analytical Methods Committee, Analyst, 2000, 125, 353
    DOI: 10.1039/A909461J

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