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Issue 7, 1998
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Holographic refractive index detector for application in microchip-based separation systems

Abstract

A novel detection scheme for capillary electrophoresis on planar glass microchips is presented. The application of a holographic-based refractive index detector to the electrophoretic separation of carbohydrates is described. The microchip device consists of a cyclic (square) separation channel having a circumference of 80 mm, a width of 40 µm and a depth of 10 µm. The volume of the injection scheme is approximately 16 pl. Separation and refractive index detection of a mixture of sucrose, N-acetylglucosamine and raffinose, each at a concentration of 33 mM, was achieved within 17 s of injection. Preliminary results demonstrate the feasibility of using hologram-based refractive index detectors in microchip separation systems. Although the initial detection limits are poor in comparison with alternative techniques, the potential of a universal detector of this kind is clear.

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Article type: Paper
DOI: 10.1039/A801478G
Analyst, 1998,123, 1443-1447

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    Holographic refractive index detector for application in microchip-based separation systems

    N. Burggraf, B. Krattiger, N. F. de Rooij, A. Manz and A. J. de Mello, Analyst, 1998, 123, 1443
    DOI: 10.1039/A801478G

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