Voltammetric behaviour of brodimoprim at mercury and its determination in biological fluids using differential-pulse polarography

Abstract

A differential-pulse polarographic (DPP) method was developed for the determination of brodimoprim (BDP) in Michaelis (MCL) buffer. The peak potential (E_p) occurs at –1.33 V (versus SCE) when the pH of the system is 4.0. The voltammetric behaviour in MCL buffer and the mechanisms of the electrode reaction were studied by, e.g., linear sweep voltammetry, cyclic voltammetry and normal-pulse polarography. The wave was confirmed to be an irreversible adsorptive wave. The relationship between peak height and concentration of BDP was linear in the range 5.0 × 10^–8–1.0 × 10^–7 mol l^–1 with a detection limit of 1.0 × 10^–9 mol l^–1, when the adsorptive accumulation time was 5 min.