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Issue 5, 1996
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Speciation of aluminium in soil extracts by employing cation-exchange fast protein liquid chromatography–inductively coupled plasma atomic emission spectrometry

Abstract

A fast protein liquid chromatographic–inductively coupled plasma (ICP) atomic emission spectrometric procedure was developed for the speciation of Al over a wide pH range, from acidic to alkaline regions. Positively charged monomeric Al species in synthetic aqueous solutions were separated from polymeric, neutral and negatively charged species on a cation-exchange fast protein LC column. Linear gradient elution with aqueous NaNO3(8 mol dm–3) at a flow rate of 1.0 cm3 min–1 over 10 min was employed for the separation. The separated Al species were determined ‘off-line’ by ICP atomic emission spectrometry in 0.5 cm3 fractions, which were diluted prior to analysis; yttrium was added as the internal standard. Retention times were found to be 1.5 min for Al(OH)2(H2O)4+[Al(OH)2+], 4.0 min for Al(OH)(H2O)52+[Al(OH)2+] and 4.5 min for Al(H2O)63+[Al3+]. Good reproducibility of measurement (relative standard deviation 1.5%) was obtained in the optimum concentration range. The limit of detection (3s) for the separated Al species was found to be 120 ng cm–3(1.0 cm3 sample). The distribution of Al species over a wide pH range agreed with the reported calculated data and, in general, agreed also with the data from the 8-hydroxyquinoline spectrophotometric method. An investigation of the effect of inorganic and organic complexing ligands on the speciation of Al indicated that positively charged [Al(SO)4+, AlF2+] and negatively charged (oxalate and citrate) Al species co-eluted with Al(OH)2+ and were separated from Al(OH)2+ and Al3+ species, while AlF2+ co-eluted with Al(OH)2+ species. At lower pH values (e.g., 4.0), aluminium citrate was partially co-eluted with Al3+ and Al(OH)2+ species. The same observations were found when soil extracts were analysed.

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Article type: Paper
DOI: 10.1039/AN9962100627
Citation: Analyst, 1996,121, 627-634

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    Speciation of aluminium in soil extracts by employing cation-exchange fast protein liquid chromatography–inductively coupled plasma atomic emission spectrometry

    B. Mitrović, R. Milačič and B. Pihlar, Analyst, 1996, 121, 627
    DOI: 10.1039/AN9962100627

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