Lucigenin immobilized on silicon oxides as a solid-phase chemiluminescent reagent

Abstract

The adsorption of an effective chemiluminescent reagent lucigenin(bis-N-methylacridinium nitrate) on different silica oxides was investigated. Adsorption of lucigenin from aqueous solutions onto non-functionalized silica gel and silpearl was found to be quick, quantitative and strong. These lucigenin-modified silicon oxide compounds were tested as potential solid-phase chemiluminescent reagents. It was found that this reagent function was stable for more then 200 analyses at a rate of 120 tests per hour. The solid-phase lucigenin reagent is irreversible but can be easily regenerated. It was applied to the flow determination of vanadium(IV) and iron(II) with detection limits of 0.7 and 0.8 µg l^–1, which are ten times more sensitive than for a closed system.