Di-2-pyridyl ketone 2-furoylhydrazone as a reagent for the fluorimetric determination of low concentrations of gallium and its application to biological samples

Abstract

Di-2-pyridyl ketone 2-furoylhydrazone (DPFH) has been examined as a sensitive spectrofluorimetric reagent for the determination of gallium A rapid procedure for the fluorimetric determination of this ion at the 2–800 ng ml^–1 level at pH 3.6–4.1 (λ_ex 395 nm, λ_em 473 nm) has been established. The detection limit is 2 ng ml^–1 and the relative standard deviation is 2.4%(40 p.p.b. of gallium). Interferences have been evaluated, and the method has been applied to the determination of gallium in biological samples (after destruction of the organic matter with HNO₃-H₂O₂).