Issue 1210, 1977

Simultaneous determination of phenobarbitone, primidone and phenytoin in small samples of blood by gas-liquid chromatography

Abstract

Phenytoin, phenobarbitone and primidone in small samples of blood can be identified and measured simultaneously. The acidified blood is extracted with 3 ml of diethyl ether and 2 ml of the extract are evaporated to dryness. The residue is dissolved in 80 µl of diethyl ether containing methyltesto-sterone (15 mg per 100 ml) as the internal standard. An aliquot of this solution is injected on to an SE-30 column where the drugs are separated and detected by a flame-ionisation detector. Drugs commonly associated in treatment with these three and cholesterol, which is co-extracted, do not interfere.

Article information

Article type
Paper

Analyst, 1977,102, 35-41

Simultaneous determination of phenobarbitone, primidone and phenytoin in small samples of blood by gas-liquid chromatography

A. M. Bruce and H. Smith, Analyst, 1977, 102, 35 DOI: 10.1039/AN9770200035

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