Determination of 3,5-dinitro-o-toluamide in feedstuffs and pre-mixes
Abstract
A spectrophotometric method is described for the determination of 3, 5-dinitro-o-toluamide (dinitolmide) in feedstuffs and pre-mixes. Dinitolmide is extracted from the sample with acetone-water (1 + 1). After purification procedures, which include liquid-liquid extraction and chromatography on an alumina column, a coloured complex is formed with sodium hydroxide, which is measured spectrophotometrically.