Direct Electrochemical Measurement of Nitric Oxide Production by Cytochrome P450-catalyzed Oxidation of N,N′-Substituted Hydroxyguanidines

Abstract

The production of nitric oxide by oxidative cleavage of N- hydroxyguanidines catalyzed by rat liver microsomes was measured directly, and for the first time by differential-pulse amperometry at a Nafion–porphyrin coated micro-sensor. The measured concentration of the nitric oxide produced (0.18–0.25 µM) was lower than the total concentration of the stored nitrites. The difference in the obtained data is discussed.