RAA-CRISPR-HCR cascade amplification for ultrasensitive visual detection of African swine fever virus DNA

Hongyu Wu; Shuhan Lin; Xiangcun Zuo; Weishi Hua; Tao Sun; Guoqing Shao; Fang Li; Dongming Zhao; Zhixin Feng; Dan Zhu; Lianhui Wang

doi:10.1039/D5CC06815K

RAA-CRISPR-HCR cascade amplification for ultrasensitive visual detection of African swine fever virus DNA

Hongyu Wu,^ab Shuhan Lin,^a Xiangcun Zuo,^a Weishi Hua,^a Tao Sun,^a Guoqing Shao,^c Fang Li,^d Dongming Zhao,^d Zhixin Feng,*^c Dan Zhu

*^ab and Lianhui Wang

*^a

Author affiliations

* Corresponding authors

^a State Key Laboratory of Flexible Electronics (LoFE) & Jiangsu Key Laboratory of Smart Biomaterials and Theranostic Technology, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China
E-mail: iamdzhu@njupt.edu.cn, iamlhwang@njupt.edu.cn

^b State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China

^c Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China
E-mail: fzxjaas@163.com

^d State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China

Abstract

An RAA-CRISPR-HCR (RCH) cascade amplification system was developed, which generates an ultrasensitive colorimetric response to low concentrations of African swine fever virus (ASFV) DNA with a detection limit of 1 copy µL⁻¹. It demonstrates excellent accuracy and specificity in real samples, facilitating efficient on-site visual early detection of ASFV.

Chemical Communications

RAA-CRISPR-HCR cascade amplification for ultrasensitive visual detection of African swine fever virus DNA

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RAA-CRISPR-HCR cascade amplification for ultrasensitive visual detection of African swine fever virus DNA

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