Simple and fast detection of CD4 and CD8 cells: integration of image flow system and acoustophoresis

Abstract

We developed a compact image-based flow cytometry system that combines acoustic focusing with machine learning-driven image analysis. The system enables rapid detection of stained CD4 and CD8 cells flowing on an acoustically defined focal plane, without the need for hydrodynamic focusing. This design allows for a compact configuration while maintaining reliable performance and reduced complexity, making the system suitable for both clinical diagnostics and point-of-care applications.