An electrochemical sensor for detecting IL-6 expression following spinal cord injury

Abstract

Based on the principle of antigen-antibody specific binding, an electrochemical sensor utilizing Prussian blue nanoparticles (PBNPs) was developed to detect the expression of the inflammatory cytokine interleukin-6 (IL-6) in vivo following spinal cord injury (SCI). The high expression of pro-inflammatory cytokines following SCI contributes to numerous pathological processes in various diseases. Experimental results demonstrated that the prepared sensor exhibited advantages such as simplicity, high stability, reproducibility, high sensitivity, high specificity, and clinical applicability. Test results from animal models indicated that the inflammatory cytokine IL-6 was rapidly and highly expressed in rats following SCI. Furthermore, comparative analysis of clinical sample test results demonstrated that this sensor exhibited high accuracy. In summary, the prepared electrochemical sensor offered an exceptionally effective approach for investigating the expression trends of post-SCI inflammatory factors. It also holds broad prospects for translational clinical testing.