Spherical nanoflower-shaped Co-2MI with laccase-like activity for colorimetric detection of salbutamol

Abstract

A Co MOF (Co-2MI) nanozyme with uniform spherical nanoflower morphology was constructed with efficient laccase-like activity. Co-2MI displayed lower K_m and higher V_max than natural Laccase, indicating stronger substrate affinity and superior catalytic efficiency. Mechanistic investigations confirmed an ˙O₂⁻-mediated oxidation pathway. Based on its laccase-like activity, we established a novel colorimetric method for Salbutamol detection, obtaining a linear range of 1–60 μg mL⁻¹ and a low detection limit of 0.91 μg mL⁻¹. Finally, the Co-2MI nanozyme was successfully applied to the colorimetric detection of Salbutamol in real samples.