Issue 97, 2025
From the journal:

Chemical Communications

CRISPR-coupled triple cascade amplification for simultaneous lateral flow detection of Mycoplasma pneumoniae and H1N1

Ruyue Wei,†a   Shuqi Wang,†a   Yingbo Pan,a   Wei Pan, ORCID logo a   Na Li ORCID logo *a  and  Bo Tang ORCID logo ab  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China
E-mail: lina@sdnu.edu.cn

b Laoshan Laboratory, Qingdao 266237, P. R. China

Abstract

We developed a CRISPR-coupled triple cascade system integrating recombinase polymerase amplification (RPA), CRISPR/Cas12a, and catalytic hairpin assembly (CHA) for simultaneous lateral flow detection of Mycoplasma pneumoniae and H1N1 in saliva samples, achieving a LOD of 10 aM for H1N1 RNA and 25 aM for MP DNA on a single LFA.

Graphical abstract: CRISPR-coupled triple cascade amplification for simultaneous lateral flow detection of Mycoplasma pneumoniae and H1N1

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Article information

DOI
https://doi.org/10.1039/D5CC04993H
Article type
Communication
Submitted
29 Aug 2025
Accepted
03 Nov 2025
First published
04 Nov 2025

Chem. Commun., 2025,61, 19241-19244

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CRISPR-coupled triple cascade amplification for simultaneous lateral flow detection of Mycoplasma pneumoniae and H1N1

R. Wei, S. Wang, Y. Pan, W. Pan, N. Li and B. Tang, Chem. Commun., 2025, 61, 19241 DOI: 10.1039/D5CC04993H

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