In operando assessment of H2O2 production by a multicopper oxidase under direct electron transfer with a gold electrode
Abstract
A deeper understanding of the intricate redox processes at biotic/abiotic interfaces requires advanced analytical tools. We present the localized and real-time characterization of a multicopper oxidase adsorbed on a gold electrode using a dedicated microbiosensor, providing detailed and spatially resolved information. Enzymatic O2 reduction is shown to proceed without detectable H2O2 accumulation. Instead, H2O2 originates from direct O2 conversion at the gold surface.