Py-Azo-decorated covalent organic frameworks as a structure-responsive mass spectrometry probe for high-sensitivity and high-throughput screening of trace toxic chemicals in complex samples

Abstract

A Py-azo-decorated covalent organic framework (Py-Azo-COF) was utilized as a structure-responsive probe for SALDI-TOF MS analysis. Benefiting from its unique structure, the Py-Azo-COF probe exhibited high affinity, sensitivity (LOD: 2–200 fg mL⁻¹), reproducibility, salt tolerance, and low background interference, enabling efficient screening of diverse trace toxic chemicals in complex samples.