In Situ Enrichment and Ultrasensitive Analysis of Interstitial Fluid miRNA Enabled by Hydrogel Microneedles Coupled with DNA-Gated Metal-Organic Frameworks

Abstract

A novel strategy combining GelMA hydrogel microneedles and DNA-gated MOFs for sensitive miRNA detection in skin interstitial fluid. GelMA MNs efficiently extract ISF, while DNA-gated MOFs offer dual-mode detection via fluorescence and SERS. In vivo results demonstrate successful miRNA extraction and sensitive biomarker detection, advancing minimally invasive diagnostics and real-time health monitoring.