Portable smartphone-enabled dydrogesterone sensors based on biomimetic polymers for personalized gynecological care

Abstract

Dydrogesterone, a frequently prescribed synthetic hormone integral to the treatment of diverse gynecological conditions, necessitates precise quantification in complex human plasma. In this study, the development of a portable, smartphone-based electrochemical sensor employing screen-printed gold electrodes (SPAuEs) modified with a biomimetic, molecularly imprinted poly(methacrylic acid-co-methyl methacrylate) (MIP) is presented for dydrogesterone detection in human plasma. FTIR spectroscopy illustrates the transformation of a pre-polymer mixture into a polymerized matrix, while SEM reveals a uniform MIP/SPAuE surface morphology. The sensor fabrication protocol, encompassing MIP/SPAuE composition, polymerization solvent, incubation time, and scan rate, is optimized to achieve enhanced sensitivity. The MIP/SPAuEs sensor exhibits a linear sensor response to dydrogesterone within the concentration range of 1–500 nM, as evidenced by cyclic and differential pulse voltammetry. The MIP/SPAuE sensor demonstrates exceptional sensitivity, recording 8.2 × 10−3 μA nM−1, with a sub-nanomolar limit of detection (LOD = 370 pM), and low limit of quantification (LOQ = 1.12 nM), along with appreciable selectivity over common interferents. In real-world clinical applications, the designed sensor is effectively employed for the rapid and precise determination of dydrogesterone in human blood plasma, achieving a remarkable recovery of 81%. Furthermore, MIP/SPAuE coatings possess suitable stability over 15 days, indicating the robustness of the sensor material for multiple rounds of analysis. The developed sensor provides a sensitive, selective, and cost-effective solution for monitoring dydrogesterone in plasma during various gynecological disorders, allowing for personalized healthcare applications.

Graphical abstract: Portable smartphone-enabled dydrogesterone sensors based on biomimetic polymers for personalized gynecological care

Article information

Article type
Paper
Submitted
28 Mar 2024
Accepted
12 Jun 2024
First published
12 Jun 2024

J. Mater. Chem. B, 2024, Advance Article

Portable smartphone-enabled dydrogesterone sensors based on biomimetic polymers for personalized gynecological care

S. Ashraf, T. Hussain, S. Z. Bajwa, A. Mujahid and A. Afzal, J. Mater. Chem. B, 2024, Advance Article , DOI: 10.1039/D4TB00657G

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