Issue 26, 2024

Multiscale analysis of triglycerides using X-ray scattering: implementing a shape-dependent model for CNP characterization

Abstract

In the last decade, research has focused on examining the fundamental interactions occurring in triglycerides, aiming to comprehend the self-assembly of crystalline nanoplatelets (CNPs) and their role in forming larger hierarchical structures essential for fat functionality. Microscopy research on CNPs frequently requires disruptive preparatory techniques, such as deoiling and sonication, to achieve quantitative outcomes. Conversely, X-ray scattering has proven to be an advantageous method for studying triglycerides, as little sample is needed to quantify the system's hierarchical structures. Specifically, ultra-small-angle X-ray scattering (USAXS) has emerged as a fitting technique for studying CNPs, owing to its length scale range falling between 25 nm and 3.49 μm. In this study, we characterized four different 30% fat dilutions of stearic acid-based fats in triolein, with various purities and preparation protocols. Samples were characterized by combining diverse microscopy techniques (cryo-SEM, TEM, polarized light and phase contrast microscopy) with synchrotron-radiation X-ray scattering (WAXS, SAXS, and USAXS). A shape-dependent model for the interpretation of USAXS data is proposed, overcoming some of the drawbacks linked to previously utilized models. CNPs are modeled as polydisperse parallelepipeds, and the aggregates are characterized by fractal dimensionality. This model offers novel insights into CNP cross-section, as well as aggregation. In the long run, we hope that the model will increase our understanding of CNP conformation and interactions, helping us design new fat systems on the mesoscale.

Graphical abstract: Multiscale analysis of triglycerides using X-ray scattering: implementing a shape-dependent model for CNP characterization

Supplementary files

Article information

Article type
Paper
Submitted
27 Feb 2024
Accepted
03 Jun 2024
First published
04 Jun 2024
This article is Open Access
Creative Commons BY license

Soft Matter, 2024,20, 5071-5085

Multiscale analysis of triglycerides using X-ray scattering: implementing a shape-dependent model for CNP characterization

I. A. Penagos, F. De Witte, T. Rimaux, W. Chèvremont, I. Pintelon, K. Dewettinck and F. Van Bockstaele, Soft Matter, 2024, 20, 5071 DOI: 10.1039/D4SM00259H

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