Volume 3, 2024

Electrochemical detection of tumor cells based on proximity labelling-assisted multiple signal amplification

Abstract

Malignant tumors are the second leading cause of human deaths worldwide, and early cancer screening and diagnosis can effectively reduce cancer mortality. Herein, we propose a new electrochemical method for the highly sensitive detection of MUC1-positive tumor cells based on proximity labelling-assisted multiple signal amplification. Specifically, a MUC1 aptamer-modified electrode was prepared for capturing MUC1-positive tumor cells, followed by binding of G4-DNA strands to the cells with the aid of a mild reduction reaction. A hemin/G4-DNA complex was then formed and acted as a mimic of horseradish peroxidase, catalysing the proximal labelling of tyramine-modified gold nanoparticles to induce silver-enhanced electrochemical signal amplification. Electrochemical results demonstrated that the method was able to specially identify MUC1-positive tumor cells and generate corresponding electrochemical responses in the range of 100 cells per mL to 1 × 106 cells per mL with a detection limit of 21 cells per mL. Furthermore, the method displayed good stability and anti-interference performance in complex serum environments. Therefore, our work may provide an effective tool to improve the accuracy of cell-based tissue examination and liquid biopsy for early diagnosis of cancers in the future.

Graphical abstract: Electrochemical detection of tumor cells based on proximity labelling-assisted multiple signal amplification

Supplementary files

Article information

Article type
Paper
Submitted
25 Jun 2024
Accepted
30 Jul 2024
First published
02 Aug 2024
This article is Open Access
Creative Commons BY-NC license

Sens. Diagn., 2024,3, 1542-1550

Electrochemical detection of tumor cells based on proximity labelling-assisted multiple signal amplification

G. Zhou, F. Zhou, X. Yu, D. Zhou, J. Wang, B. Bo, Y. Cao and J. Zhao, Sens. Diagn., 2024, 3, 1542 DOI: 10.1039/D4SD00217B

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