Issue 11, 2024

In situ SERS imaging of protein-specific glycan oxidation on living cells to quantitatively visualize pathogen–cell interactions

Abstract

Glycan oxidation on the cell surface occurs in many specific life processes including pathogen–cell interactions. This work develops a surface-enhanced Raman scattering (SERS) imaging strategy for in situ quantitative monitoring of protein-specific glycan oxidation mediated pathogen–cell interactions by utilizing Raman reporter DTNB and aptamer co-assembled platinum shelled gold nanoparticles (Au@Pt-DTNB/Apt). Using Fusarium graminearum (FG) and MCF-7 cells as models, Au@Pt-DTNB/Apt can specifically bind to MUC1 protein on the cell surface containing heavy galactose (Gal) and N-acetylgalactosamine (GalNAc) modification. When FG interacts with cells, the secreted galactose oxidase (GO) can oxidize Gal/GalNAc, and the generated reactive oxygen species (ROS) further oxidizes DTNB to produce TNB for greatly enhancing the SERS signal. This strategy can quantitatively visualize for the first time both the protein-specific glycan oxidation and the mediated pathogen–cell interactions, thus providing key quantitative information to distinguish and explore the pathogen-resistance and pharmacological mechanisms of different drugs.

Graphical abstract: In situ SERS imaging of protein-specific glycan oxidation on living cells to quantitatively visualize pathogen–cell interactions

Supplementary files

Article information

Article type
Edge Article
Submitted
09 Jan 2024
Accepted
30 Jan 2024
First published
02 Feb 2024
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2024,15, 3901-3906

In situ SERS imaging of protein-specific glycan oxidation on living cells to quantitatively visualize pathogen–cell interactions

Y. Wang, S. Wu, Y. Yang, Y. Yang, H. Liu, Y. Chen and H. Ju, Chem. Sci., 2024, 15, 3901 DOI: 10.1039/D4SC00157E

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