Simple and direct electrochemical detection of rosmarinic acid in food samples based on nanochannel modified carbon electrode

Abstract

The detection of rosmarinic acid (Ros A) in food samples holds major significance. Simple and convenient electrochemical detection of Ros A with high performance remains a challenge. In this work, a nanochannel array-modified carbon electrode was constructed using a simple and convenient approach to achieve highly sensitive electrochemical detection of Ros A in food samples. Through simple electrochemical pre-activation of a glassy carbon electrode (GCE), oxygen-containing functional groups were introduced on the electrode surface (p-GCE). Vertically-ordered mesoporous silica film (VMSF) was stably grown on p-GCE through electrochemical-assisted self-assembly (EASA) without the introduction of another adhesive layer (VMSF/p-GCE). Transmission electron microscopy (TEM) characterization demonstrated the highly ordered structure of VMSF with a nanochannel diameter around 2.7 nm. Both p-GCE and the nanochannels significantly enhanced the electrochemical signals of Ros A on the electrode, exhibiting dual signal amplification. VMSF/p-GCE demonstrated sensitive detection of Ros A with a linear range of 500 nM to 1 μM and 1 μM to 35 μM. The detection limit (DL) was 26 nM. Combining the good anti-fouling and anti-interference properties of the nanochannels, VMSF/p-GCE can achieve direct electrochemical detection of Ros A in food samples. The sensor can be easily regenerated for repeated use. The simple fabrication, high detection sensitivity and selectivity of the sensor make it a new strategy for rapid preparation of high-performance electrochemical sensors.