A phosphamide nucleotide analog: a substrate for polymerase synthesis of DNA

Abstract

A type of modified nucleotide, deoxynucleotide γ-amidotriphosphates (dNTPγNH₂s), exhibited around five times higher stability than dNTPs. These phosphamide nucleotides can be utilized by several DNA polymerases, and the amplification of a 10 kb DNA fragment through the polymerase chain reaction (PCR) can be accomplished even under conditions of high temperature, extended storage, or repeated freeze–thaw cycles. However, the control PCR with standard dNTPs was unsuccessful. These results indicate that dNTPγNH₂s have the potential to substitute dNTPs in PCR.