Issue 15, 2024

Toll like receptor-based electrochemical sensors via N-heterocyclic carbene-modified surfaces: towards improved sensing of DNA molecules

Abstract

Pathogen detection on gold surfaces using N-heterocyclic carbenes (NHCs) functionalized with amine tags along with an organic linker, bis(sulfosuccinimidyl)suberate sodium salt (BS3) to chemically bind toll-like receptor 9 (TLR9) to gold electrodes, is reported here for the first time. TLRs are essential components of the early warning system of the human innate immune response, with TLR9 being a DNA sensing receptor that specifically detects DNA viruses. To enhance sensor consistency compared to previous literature, two distinct types of NHCs were used to minimize challenges associated with non-specific adsorption. Gold electrodes functionalized with TLRs via NHCs have the potential to operate as broad-spectrum biosensors, as evidenced by their ability to identify biological pathogens based on alterations in resistance and pattern recognition.

Graphical abstract: Toll like receptor-based electrochemical sensors via N-heterocyclic carbene-modified surfaces: towards improved sensing of DNA molecules

Supplementary files

Article information

Article type
Paper
Submitted
23 Feb 2024
Accepted
17 Apr 2024
First published
18 Apr 2024
This article is Open Access
Creative Commons BY-NC license

Mater. Adv., 2024,5, 6063-6069

Toll like receptor-based electrochemical sensors via N-heterocyclic carbene-modified surfaces: towards improved sensing of DNA molecules

D. S. Lee, M. P. Zarabadi, H. Bhattacharjee, L. Qi, J. F. McLeod, K. Saeedfar, I. Singh, A. Woods, A. Messina, V. I. Birss, C. M. Crudden and Z. She, Mater. Adv., 2024, 5, 6063 DOI: 10.1039/D4MA00188E

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements