Issue 19, 2024

A smartphone-based immunochromatographic strip platform for on-site quantitative detection of antigenic targets

Abstract

To report the testing signal of an immunochromatographic assay for on-site quantitative detection, a portable and user-friendly smartphone-based biosensing platform is developed in this study. This innovative system is composed of an ambient light sensor inherent smartphone reader and a 3D-printed handhold device, a quantitative tool capable of directly interpreting carbon nanoparticle (CNP)-conjugated immunochromatographic strips. To showcase the platform capability, the smartphone-based immunochromatography system (SPICS) reader and device were successfully used in CNP strips for rapid detection of the early pregnancy marker human chorionic gonadotropin in female urine (HCG; limit of detection [LOD]: 0.30 mIU mL−1), prostate-specific antigen in patient blood (PSA; LOD: 0.28 ng mL−1) and ampicillin residue in animal milk (AMP; LOD: 0.23 ng mL−1). The results were fully correlated with conventional commercial instruments (R2 = 0.99). The SPICS platform exhibits significant advantages, including portability, cost-effectiveness, easy operation, and rapid and quantitative detection, making it a valuable on-site diagnosis tool for use in home and community healthcare facilities.

Graphical abstract: A smartphone-based immunochromatographic strip platform for on-site quantitative detection of antigenic targets

Supplementary files

Article information

Article type
Paper
Submitted
02 Jun 2024
Accepted
17 Aug 2024
First published
19 Aug 2024

Lab Chip, 2024,24, 4639-4648

A smartphone-based immunochromatographic strip platform for on-site quantitative detection of antigenic targets

E. Zhang, Q. Zeng, Y. Xu, J. Lu, C. Li, K. Xiao, X. Li, J. Li, T. Li, C. Li and L. Zhang, Lab Chip, 2024, 24, 4639 DOI: 10.1039/D4LC00484A

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