Issue 46, 2024

DNAzyme-activated CRISPR/Cas assay for sensitive and one-pot detection of lead contamination

Abstract

Hazardous lead ions (Pb2+) even at a minute level can pose side effects on human health, highlighting the need for tools for trace Pb2+ detection. Herein, we present a DNAzyme-activated CRISPR assay (termed DzCas12T) for sensitive and one-pot detection of lead contamination. Using an extension-bridged strategy eliminates the need for separation to couple the DNAzyme recognition and CRISPR reporting processes. The tandem design endowed the DzCas12T assay with high specificity and sensitivity down to the pM-level. This assay has been used to detect lead contamination in food and water samples, indicating the potential for monitoring lead-associated environmental and food safety.

Graphical abstract: DNAzyme-activated CRISPR/Cas assay for sensitive and one-pot detection of lead contamination

Supplementary files

Article information

Article type
Communication
Submitted
19 Apr 2024
Accepted
14 May 2024
First published
15 May 2024

Chem. Commun., 2024,60, 5976-5979

DNAzyme-activated CRISPR/Cas assay for sensitive and one-pot detection of lead contamination

R. Deng, Y. Bai, Y. Liu, Y. Lu, Z. Zhao, Y. Deng and H. Yang, Chem. Commun., 2024, 60, 5976 DOI: 10.1039/D4CC01852D

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