Issue 40, 2024

Biphasic water–oil systems for functional augmentation of probiotic Lactobacillus acidophilus nanoencapsulated in luteolin-Fe3+ shells

Abstract

Single-cell nanoencapsulation (SCNE) has great potential in the enhancement of therapeutic effects of probiotic microbes. However, the material scope has been limited to water-soluble compounds to avoid non-biocompatible organic solvents that are harmful to living cells. In this work, the SCNE of probiotic Lactobacillus acidophilus with water-insoluble luteolin and Fe3+ ions is achieved by the vortex-assisted, biphasic water–oil system. The process creates L. acidophilus nanoencapsulated in the luteolin-Fe3+ shells that empower the cells with extrinsic properties, such as resistance to lysozyme attack, anti-ROS ability, and α-amylase-inhibition activity, as well as sustaining viability under acidic conditions. The proposed protocol, embracing water-insoluble flavonoids as shell components in SCNE, will be an advanced add-on to the chemical toolbox for the manipulation of living cells at the single-cell level.

Graphical abstract: Biphasic water–oil systems for functional augmentation of probiotic Lactobacillus acidophilus nanoencapsulated in luteolin-Fe3+ shells

Supplementary files

Article information

Article type
Communication
Submitted
08 Apr 2024
Accepted
19 Apr 2024
First published
22 Apr 2024

Chem. Commun., 2024,60, 5330-5333

Biphasic water–oil systems for functional augmentation of probiotic Lactobacillus acidophilus nanoencapsulated in luteolin-Fe3+ shells

D. T. Nguyen, S. Y. Han, F. Kozlowski, G. A. Seisenbaeva, V. G. Kessler, B. J. Kim and I. S. Choi, Chem. Commun., 2024, 60, 5330 DOI: 10.1039/D4CC01603C

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