Unexpected enrichment of DNA aptamers for Zn2+ ions from an insulin selection

Yibo Liu; Xiaoqin Wang; Juewen Liu

doi:10.1039/D4CC01546K

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Unexpected enrichment of DNA aptamers for Zn²⁺ ions from an insulin selection†

Yibo Liu,^ab Xiaoqin Wang^a and Juewen Liu

*^ab

Author affiliations

* Corresponding authors

^a Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, Canada
E-mail: liujw@uwaterloo.ca

^b Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Hong Kong, China

Abstract

We serendipitously discovered Zn²⁺-binding DNA aptamers when selecting insulin aptamers. The Zn-1 aptamer binds to Zn²⁺ with a dissociation constant (K_d) of ∼1 μM, and has 450-fold higher selectivity for Zn²⁺ over Cd²⁺. A strand-displacement based fluorescent sensor achieved a limit of detection of 0.2 μM Zn²⁺.

Download options Please wait...

Supplementary files

Supplementary information PDF (925K)

Article information

DOI: https://doi.org/10.1039/D4CC01546K
Article type: Communication
Submitted: 03 Apr 2024
Accepted: 23 May 2024
First published: 24 May 2024

Download Citation

Chem. Commun., 2024,60, 6280-6283

Permissions

Request permissions

Unexpected enrichment of DNA aptamers for Zn²⁺ ions from an insulin selection

Y. Liu, X. Wang and J. Liu, Chem. Commun., 2024, 60, 6280 DOI: 10.1039/D4CC01546K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications