Steric hindrance of N6-methyl in m6A and its application for specific loci detection

Xiaoyi Xie; Qi Wei; Jingyu Han; Xin Fang; Wei Yang; Xiang Zhou; Yafen Wang; Xiaocheng Weng

doi:10.1039/D4CC01041H

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Steric hindrance of N⁶-methyl in m⁶A and its application for specific loci detection†

Xiaoyi Xie,‡^a Qi Wei,‡^a Jingyu Han,‡^a Xin Fang,^a Wei Yang,^a Xiang Zhou,

^a Yafen Wang*^b and Xiaocheng Weng

*^a

Author affiliations

* Corresponding authors

^a College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei, China
E-mail: xcweng@whu.edu.cn

^b School of Public Health, Wuhan University, Wuhan 430071, Hubei, China
E-mail: yfwang@whu.edu.cn

Abstract

We found that the N⁶ methyl group of N⁶-methyladenine is able to hinder the methylation of N⁶-methyladenine at the N¹ position by DMS. Based on this, we have devised a novel method for detecting N⁶-methyladenine, which was successfully applied to identify specific m⁶A loci in 28S rRNA.

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Article information

DOI: https://doi.org/10.1039/D4CC01041H
Article type: Communication
Submitted: 05 Mar 2024
Accepted: 20 Mar 2024
First published: 21 Mar 2024

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Chem. Commun., 2024,60, 4479-4482

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Steric hindrance of N⁶-methyl in m⁶A and its application for specific loci detection

X. Xie, Q. Wei, J. Han, X. Fang, W. Yang, X. Zhou, Y. Wang and X. Weng, Chem. Commun., 2024, 60, 4479 DOI: 10.1039/D4CC01041H

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Chemical Communications