Issue 35, 2024

One-pot isothermal amplification permits recycled activation of CRISPR/Cas12a for sensing terminal deoxynucleotidyl transferase activity

Abstract

This study introduces a one-pot isothermal amplification assay for ultrasensitive analysis of terminal deoxynucleotidyl transferase (TdT) activity. The system realizes recycled activation of CRISPR/Cas12a, enabling exceptional signal amplification. This approach maximizes the simplicity of the detection method, offering a promising avenue for molecular disease diagnosis.

Graphical abstract: One-pot isothermal amplification permits recycled activation of CRISPR/Cas12a for sensing terminal deoxynucleotidyl transferase activity

Supplementary files

Article information

Article type
Communication
Submitted
20 Feb 2024
Accepted
02 Apr 2024
First published
02 Apr 2024

Chem. Commun., 2024,60, 4683-4686

One-pot isothermal amplification permits recycled activation of CRISPR/Cas12a for sensing terminal deoxynucleotidyl transferase activity

J. Xu, H. Yang, Z. Sui, X. Yuan, L. Jia and L. Guo, Chem. Commun., 2024, 60, 4683 DOI: 10.1039/D4CC00825A

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