Simultaneous and multiplexed phenotyping of circulating exosomes with the orthogonal CRISPR–Cas platform

Gaoxing Su; Mengting Xu; Yuedong Zhu; Yan Zhang; Yanan Lin; Yanyan Yu

doi:10.1039/D4CC00497C

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Simultaneous and multiplexed phenotyping of circulating exosomes with the orthogonal CRISPR–Cas platform†

Gaoxing Su,

^a Mengting Xu,^ab Yuedong Zhu,^a Yan Zhang,^a Yanan Lin^a and Yanyan Yu

*^a

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* Corresponding authors

^a School of Pharmacy, Nantong University, Nantong, Jiangsu 226001, China
E-mail: yuyanyan@ntu.edu.cn

^b Department of Pharmacy, Yixing Fifth People's Hospital, Yixing, Jiangsu, China

Abstract

Simultaneous and multiplexed exosome protein profiling via an orthogonal CRISPR–Cas platform was achieved in this work. Aptamers were recruited to translate exosome surface protein information into Cas12a/Cas13a cleavage activity. The established multiplexed platform performed robustly with biological matrixes and could profile exosome proteins in clinical serum samples.

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Article information

DOI: https://doi.org/10.1039/D4CC00497C
Article type: Communication
Submitted: 01 Feb 2024
Accepted: 04 May 2024
First published: 20 May 2024

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Chem. Commun., 2024,60, 5944-5947

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Simultaneous and multiplexed phenotyping of circulating exosomes with the orthogonal CRISPR–Cas platform

G. Su, M. Xu, Y. Zhu, Y. Zhang, Y. Lin and Y. Yu, Chem. Commun., 2024, 60, 5944 DOI: 10.1039/D4CC00497C

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Chemical Communications

Simultaneous and multiplexed phenotyping of circulating exosomes with the orthogonal CRISPR–Cas platform†

Abstract

Supplementary files

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Simultaneous and multiplexed phenotyping of circulating exosomes with the orthogonal CRISPR–Cas platform

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