Turn-on fluorescence detection of carbon monoxide in plant tissues based on Cu2+ modulated polydihydroxyphenylalanine nanosensors

Abstract

As an important signaling molecule, carbon monoxide (CO) plays an important role in plant growth and development including affecting stomatal movement, stress response and root development. Thus, it is necessary to develop fluorescent probes that can be used to detect CO in live plant tissues and further enable a deep-understanding of its biological function, mechanism and metabolism. In this paper, a novel and sensitive fluorescent probe based on Cu²⁺ modulated polydihydroxyphenylalanine nanoparticles (PDOAs) has been developed for the detection of CO. The fluorescence of PDOAs can be effectively quenched by Cu²⁺ through the multi-coordination interaction. In the presence of CO, Cu²⁺ can be effectively reduced to Cu⁺, which resulted in the release of free PDOAs and the Cu²⁺-quenched bright green fluorescence was restored obviously. Through this ingenious strategy, the abiotic CO can be accurately detected and identified with high selectivity, rapid response time within 5 min and an ultralow detection limit of 72.4 nM. Due to the admirable biocompatibility, the nano-material based probe has been successfully applied for in vivo imaging CO in the root tip and leave tissues of lettuce. To the best of our knowledge, this is the first example of a fluorescent probe-based methodology for the sensitive tracking of CO in plant tissues.