An in situ ratiometric fluorescence immunosensor via Mn2+-triggered aggregation-induced emission transformation of levodopa fluorescent copolymer nanoparticles

Yifei Ma; Xiaoxue Liu; Naidi Yang; Changpeng Zou; Yujie Sun; Guichuan Xing; Jinhua Liu; Zhihui Xu; Fenghua Geng

doi:10.1039/D4AY00973H

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An in situ ratiometric fluorescence immunosensor via Mn²⁺-triggered aggregation-induced emission transformation of levodopa fluorescent copolymer nanoparticles†

Yifei Ma,‡^a Xiaoxue Liu,‡^a Naidi Yang,

‡^a Changpeng Zou,^a Yujie Sun,^a Guichuan Xing,

^a Jinhua Liu,

*^a Zhihui Xu*^c and Fenghua Geng

*^b

Author affiliations

* Corresponding authors

^a Key Laboratory of Flexible Electronics (KLOFE), Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, PR China
E-mail: iamjhliu@njtech.edu.cn

^b Henan Key Laboratory of Chemo/Biosensing and Chemometrics, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, School of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, China
E-mail: gengfh@126.com

^c Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, P. R. China
E-mail: wx-xzm@163.com

Abstract

Herein, a direct in situ alkaline phosphatase (ALP)-labeled luminescent nanoimmunoassay platform was constructed using Mn²⁺-triggered aggregation-induced emission transformation of levodopa fluorescent copolymer (LFC) nanoparticles. Using cardiac troponin I (cTn I) as the model antigen, the proposed nanoimmunosensor has been applied to detect cTn I in clinical samples with satisfactory results.

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Article information

DOI: https://doi.org/10.1039/D4AY00973H
Article type: Communication
Submitted: 24 May 2024
Accepted: 27 Aug 2024
First published: 10 Sep 2024

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Anal. Methods, 2024,16, 6676-6680

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An in situ ratiometric fluorescence immunosensor via Mn²⁺-triggered aggregation-induced emission transformation of levodopa fluorescent copolymer nanoparticles

Y. Ma, X. Liu, N. Yang, C. Zou, Y. Sun, G. Xing, J. Liu, Z. Xu and F. Geng, Anal. Methods, 2024, 16, 6676 DOI: 10.1039/D4AY00973H

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