Issue 26, 2024

Bioluminescent aptamer-based microassay for detection of melanoma inhibitory activity protein (MIA)

Abstract

Melanoma inhibitory activity protein (MIA) does obviously offer the potential to reveal clinical manifestations of melanoma. Despite a pressing need for effective diagnosis of this highly fatal disease, there are no clinically approved MIA detection ELISA kits available. A recommended MIA threshold has not yet been defined, mostly by reason of variability in immunoglobulins' affinity and stability, the difference in sample preparation and assay conditions. Here we present a pair of high-affinity DNA aptamers developed as an alternative recognition and binding element for MIA detection. Their stability and reproducible synthesis are expected to ensure this analysis under standard conditions. The devised aptamer-based solid-phase microassay of model standard and control human sera involves luciferase NLuc as a highly sensitive reporter. Bioluminescence dependence on MIA concentration ranges in a linear manner from 2.5 to 250 ng mL−1, providing a MIA detection limit of 1.67 ± 0.57 ng mL−1.

Graphical abstract: Bioluminescent aptamer-based microassay for detection of melanoma inhibitory activity protein (MIA)

Supplementary files

Article information

Article type
Communication
Submitted
18 Apr 2024
Accepted
11 Jun 2024
First published
20 Jun 2024

Anal. Methods, 2024,16, 4234-4239

Bioluminescent aptamer-based microassay for detection of melanoma inhibitory activity protein (MIA)

E. E. Bashmakova, A. N. Kudryavtsev, A. E. Tupikin, M. R. Kabilov, A. E. Sokolov and L. A. Frank, Anal. Methods, 2024, 16, 4234 DOI: 10.1039/D4AY00706A

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