A highly effective peroxidase-mimic nanozyme of S, N-carbon dot-decorated cerium organic framework-based colorimetric detection of Hg2+ ion and thiophanate methyl

Abstract

In this study, we proposed a colorimetric probe as S, N-carbon dot-decorated Ce-MOF (S, N-CD@Ce-MOF) for the dual detection of mercury and thiophanate methyl (TM), which are simultaneously present pollutants in the environment and foodstuffs. These pollutants cause serious threats to human health, such as carcinogenicity and neurovirulence. Herein, we synthesized S, N-CD@Ce-MOF using the hydrothermal method and applied it to a “turn-off–on” probe to detect mercury and TM using the colorimetric method in water and food samples. S, N-CD@Ce-MOF shows excellent peroxidase activity by catalyzing the chromogenic substrate of 3,3′,5,5′-tetramethylbenzidine (TMB), resulting in deep blue-colored oxidized TMB product (ox TMB) in the presence of H₂O₂ with a UV absorption wavelength at 654 nm. However, the addition of Hg(II) ions prohibits the oxidation of TMB by an electron transfer effect and easily binds with –S, –N-containing sites on the surface of carbon dots, obstructing the catalytic active sites and decreasing catalytic efficiency with weak UV absorption at 654 nm as a “turn-off”. Subsequently, the addition of TM to the above sensing solution as a “turn-on” was triggered by the TM–Hg complex formation and permitted TMB oxidation with a strong absorption peak at 654 nm. Furthermore, this proposed sensor demonstrates a superior linear response to mercury ions and TM in the ranges from 0 to 15 μM and 0 to 14 μM, respectively. The developed colorimetric assay exhibits good sensitivity and selectivity against various possible interferences. Furthermore, we found that the limits of detection for Hg²⁺ and TM were as low as 0.01 μM and 0.03 μM, respectively. The developed sensor provides various benefits, such as cost-effectiveness, simplicity without a complex detection process, and naked-eye detection. Consequently, our proposed colorimetric technique worked well for the detection of Hg²⁺ in real water samples and TM in real apple and tomato juice.