Issue 9, 2024
From the journal:

Analyst

Single-virus-sensitive barcode qPCR mediated by the aggregation of gold nanoparticle probes†

Yuanzhao Shen, ORCID logo a   Chih-Tsung Yang,b   Weiwei Li*c  and  Xin Zhou ORCID logo *a  

* Corresponding authors

a College of Veterinary Medicine, Institute of Comparative Medicine, Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China
E-mail: zhou_xin@126.com

b Future Industries Institute, University of South Australia, Mawson Lakes Blvd, Mawson Lakes, South Australia 5095, Australia

c Institute of Pediatrics, Children's Hospital of Fudan University, Fudan University, Shanghai 201102, China
E-mail: weiweili@fudan.edu.cn

Abstract

Herein, we developed a gold nanoparticle (GNP)-mediated barcode qPCR strategy with a sensitivity for a single virus particle per reaction for the detection of influenza virus H3N2. The analysis of the results for pure virus and real virus samples show that GNP-mediated barcode qPCR is ∼16 times more sensitive than conventional qPCR, demonstrating the potential to reduce false negatives and improve early diagnosis of viral infections.

Graphical abstract: Single-virus-sensitive barcode qPCR mediated by the aggregation of gold nanoparticle probes

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Article information

DOI
https://doi.org/10.1039/D4AN00121D
Article type
Communication
Submitted
24 Jan 2024
Accepted
17 Mar 2024
First published
18 Mar 2024

Analyst, 2024,149, 2556-2560

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Single-virus-sensitive barcode qPCR mediated by the aggregation of gold nanoparticle probes

Y. Shen, C. Yang, W. Li and X. Zhou, Analyst, 2024, 149, 2556 DOI: 10.1039/D4AN00121D

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