Multidrug efflux pumps excrete a range of small molecules from bacterial cells. In this study, we show that bacterial efflux pumps have affinity for a range of SYTO™ dyes that are commonly used to label bacteria. Efflux pump activity will there lead to false negative results from bacterial staining and SYTO™ dyes should be used with caution on live samples.
G. A. S. Minero, P. B. Larsen, M. E. Hoppe and R. L. Meyer, Analyst, 2024, 149, 2232 DOI: 10.1039/D3AN02112B
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